Mutations of the EGFR Gene in Patients with Non-smallCell Lung Cancer Belonging to a Population in Western Algeria.
Mutations of the EGFR Gene in Patients with Non-smallCell Lung Cancer Belonging to a Population in Western Algeria.
| dc.contributor.author | TEMIMI LINDA | |
| dc.date.accessioned | 2025-12-07T11:58:24Z | |
| dc.date.available | 2025-12-07T11:58:24Z | |
| dc.date.issued | 2025-11-22 | |
| dc.description.abstract | Worldwide, lung cancer ranks first in terms of incidence and mortality. In Algeria, this cancer represents the third most commonform of cancer afte rbreast and colorectal cancers and the second cause of death after breast cancer. In this workour objective was to comprehensive lycharacterize the epidemiological and molecular profile of lung cancer in western Algeria. The molecular analysis involved a case-control study investigatingtwo EGFR polymorphisms (rs2072454 and rs2227983) (via PCR-RFLP) and an observational retrospective study analysing EGFR mutations (via real-time PCR) in NSCLC patients. First, wecarried out an in-depth epidemiological analysis of 312 medical records which revealed a predominance of men and smokers (87% and 83% respectively). Notably, female patients were overwhelmingly non-smokers (98%) and predominantly developed ADC (88%). Significant associations were identified for tobacco and cannabis use (p<0.05), but not alcohol consumption. Occupational exposures in construction and industrial sectorswas the most frequent constituting potential risk factors among male patients. Comorbidities were presentin 56% of patients, with hypertension (23%) and diabetes (13%) being most prevalent. A family history of cancer was reported in 25% of cases, showing a significant correlation with age at diagnosis (p<0.005), suggesting potential hereditary predisposition patterns. Second, our case-control study involving 143 participants (73 lung cancer cases and 70 healthycontrols) demonstrated that the EGFR rs2072454-CT genotype conferred a 2.35-fold increasedrisk of lung cancer (95% CI 1.04-5.30, p=0.04), while the rs2227983-AG variant wasspecifically associated with a 2.76-fold elevated ADC risk (95% CI 1.0-7.5, p=0.04). Third, weperformed a molecular profiling of 71 NSCLC FFPE tissue specimens that identified EGFR mutations in 28% of cases (72% wild-type), with 60% exhibiting single mutations (predominantly L858R [n=7], followed by S768I [n=3] and L861Q [n=2]) and 40% displaying compound mutation patterns, including four distinct double-mutation combinations (T790M+L861Q [n=1], DEL+L858R [n=2], L861Q+G719X [n=1], L861Q+S768I [n=1]) and two triple-mutation patterns (L858R+L861Q+G719X [n=1], DEL+L858R+L861Q [n=2]). These comprehensive findings underscore the criticalneed for implementing targeted lung cancer screening programs and routine EGFR mutation testing to guide personalizedtherapeuticstrategies for Algerian patients, whilehighlighting distinct epidemiological patterns and molecular characteristicsthatmayinform future research and public health interventions in NorthAfrican populations. | |
| dc.format | ||
| dc.identifier.uri | https://dspace.univ-oran1.dz/handle/123456789/4850 | |
| dc.language.iso | en | |
| dc.publisher | Université Oran1 | |
| dc.subject | lung cancer; epidemiology; rs2072454; rs2227983; EGFR; NSCLC; PCR-RFL; real time PCR; tobacco; cannabis. | |
| dc.title | Mutations of the EGFR Gene in Patients with Non-smallCell Lung Cancer Belonging to a Population in Western Algeria. | |
| dc.type | Thesis | |
| grade.Co-rapporteur | BOURAS NORIA, MCA, Université USTO-MB | |
| grade.Examinateur | BELHADJ Amina, MCA, Université Oran 1 | |
| grade.Examinateur | ABDERAHMANE Rym, Professeur, USTO-MB | |
| grade.Grade | Doctorat | |
| grade.Option | Biologie et Pathologie Cellulaire | |
| grade.Président | SEDDIKI Sonia Amal, Professeur, Université Oran 1 | |
| grade.Rapporteur | SAHRAOUI TEWFIK, Professeur, Université Oran 1 | |
| l'article.1.DateParution | 09 May2025 | |
| l'article.1.Revue | African Journal of Biological Sciences | |
| l'article.1.Référence | Volume 7, Issue 5,May 2025 | |
| l'article.1.Titre | Epidemiological Insights into Lung Cancer in Western Algeria: A Cross-Sectional Study of Patient Characteristics and Risk Factors | |
| la.Mention | Très Honorables | |
| la.Spécialité | Biologie | |
| la.cote | TH5625 |
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