Adsorption of BSA protein on silicon, germanium and titanium dioxide investigted by in situ ATR-IR spectroscopy
Adsorption of BSA protein on silicon, germanium and titanium dioxide investigted by in situ ATR-IR spectroscopy
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Date
2013-03-11
Auteurs
BOUHEKKA Ahmed
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Éditeur
Université Oran1 Ahmed Ben Bella
Résumé
The overall goal of this research effort was to the study the adsorption of Bovine Serum Albumin protein onto different surfaces like: Silicon, Germanium and Titanium Dioxide using in situ attenuated total reflection spectroscopy. Important parameters for adsorption like pH, temperature and ionic strength are studied to evaluate their effect on adsorption. One of the most important points in this research was to study the photo-degradation of BSA onto TiO2 surface by UV illumination and to examine the changes in the secondary structure of adsorbed BSA using curve fitting of the second derivative which allows a direct quantitative analysis. Another point was to study the effect of visible light irradiation on the secondary structure of BSA during rinsing with water.
Description
Mots-clés
Semi-conductors, Silicon, Germani, um, Titanium Dioxide, Photocatalysis, Attenuated Total Reflection Infrared Spectroscopy, Protein Adsorption, Solid-Liquid Interface, Protein Denaturation, Protein Secondary Structure